Workshop on “Applied Microbiology, Molecular Biology & Bioinformatics




Center for Health and Disease Studies – Nepal (CHDS-Nepal) welcomes you all to participate in the most engaging workshop which is going to train you for the career most tools in your life science and research. 

Workshop on

Applied Microbiology, Molecular Biology & Bioinformatics

Individual hands on practical on

  1. Isolation of bacteria, Pure Culture, Biochemical tests, Antibiotic Susceptibility Test
  2. DNA extraction: Genomic & Plasmid (Bacteriak and Blood),Manual & Kit method)
  3. Conventional PCR (Gradient) and troubleshooting
  4. Restriction Digestion, and their mapping
  5. Competent cell preparation and transformation
  6. Recombinant DNA Technology (RDT)
  7. Agarose Gel Electophoresis
  8. Practical Bioinformatics: (Data retrieval, sequence alignment, BLAST, Construction of phylogenetic tree, primer design and check)

Date: 9-13 July 2018  (२५ – २९ आषाढ २०७५)

Time: 10:30 am – 4:30 pm


  • NRs. 11,000 per person for professionals
  • NRs. 10,000 per person for students 


Location Map:

Contacts:   CHDS-Nepal, Shakhamul-31,Baneshwor;

9841744582; 980211703

Microbiology, Molecular Biology techniques and Bioinformatics tools have significant values in basic as well as applied biomedical research. Microbes are present everywhere possessing both useful and harmful characters in them which is coded as their genetic material. The proper isolation, culture and identification of microbes is preliminary step in diagnosis of diseases, characterization of virulence and useful properties and further processing and manipulation. After the discovery of DNA, molecular biology has become one of the powerful tools in biomedical research not only to investigate the basic science questions, but also in understanding the wide variety of genotypic and phenotypic traits of human diseases. Molecular biology techniques such
as Polymerase Chain reaction (PCR) and protein analyses are the most widely used confirmatory techniques for diagnosis of various diseases due to their high specificity and sensitivity. Hence, the knowledge of microbiology, molecular biology and bioinformatics module are indispensable to life sciences and medical students. Life sciences students should be hands-on trained in these techniques starting from their undergraduate level to be competitive in the job market in the national as well as in the international arena. Therefore, understanding the basic principles and techniques of microbiology, molecular biology and bioinformatics are very important to make the career in biomedical or life sciences. With the rapid development of tools in biomedical research, molecular biology well tags with a fairly new branch of science, called Bioinformatics.

Bioinformatics tools can be used to explore every aspect of not only the individual genes and proteins but also a whole genome, transcriptome or proteome. This can be used to study the sequence of the gene, its translational status, structure and functions and associated metabolic pathways. Moreover, at present, bioinformatics is widely used in biomedical research for identification of novel genes, gene to disease association, prediction of biomarkers, understanding
the epigenome, expression status of a gene and so on. Hence, life science students, in addition to bench work, should be familiar with the basic tools and techniques of bioinformatics and their application, so that they are competent enough to use Molecular Biology in the most appropriate
way along with bioinformatics tool.
This training program designed by CHDS-Nepal is aimed at educating Bachelors and Masters students of biotechnology, microbiology, biochemistry and medical laboratory technology and research scholar. The training designed by CHDS-Nepal is based on experiences gained by CHDS-Nepal team while training and supervising Bachelors and Masters students in western Universities.

A brief view on the training module is given below.

Aims and outcomes:
To give hands-on practical training in the applied microbiology, molecular biology techniques, together with an introduction and application of bioinformatics. The module also aims to provide theoretical knowledge on the application of microbiology, molecular biology and bioinformatics in biomedical research and clinical diagnosis.
Outcomes By the end of this module, students should be able to
• Perform microbiology, molecular biology techniques
• Understand the principle of these techniques
• Understand their use in research and disease diagnosis
• Learn bioinformatics tools and its applications in molecular

 The module is expected to include sessions addressing the following topics
• Lab Safety
• Instrumentation and techniques
• Microbes (Bacteria, Fungi) and their isolation and identification
• Antibiotic Susceptibility Test
• DNA, Plasmid extraction
• PCR amplification
• Restriction digestion
• Competent Cell preparation, Transformation
• Bioinformatics tools including designing primers and vector maps and other genomics and proteomics tools.


Posted In: Trainings and CareersBioinformaticsCHDSCHDS-NepalMicrobiologyMolecular BiologyWorkshop Tags: , , , , , June 1st, 2018

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