Prajita Thapa Magar
My name is Prajita Thapa Magar. I have completed my Master’s degree in M.Sc. Public Health Microbiology from St.Xavier’s College, Maitighar, Kathmandu I am currently doing my thesis entitled “Isolation, screening and molecular detection of bacteria isolated from solid waste management worker” in Centre for health and disease studies Nepal, Kathmandu. I am also guiding bachelor’s level intern in their biofilm project.
After completion of my undergraduate level from Kathmandu University in B. Tech in Biotechnology, I am currently part of CHDS organization as a research intern. I am engaged in few of the project on-going in the organization. I perform molecular characterization of micro of biofilm generating population of non-multidrug resistant E. coli. I am also involved in analysis of EGFR mutation in non-small cell lung carcinoma. Here, I perform microbial isolation, antibiotic suspectibility test and molecular characterization using PCR techniques and different bioinformatics tools.
In free time, I love watching football, cricket and learning programming languages.
On April, 2019, I joined Centre for Disease and Health Studies- Nepal (CHDS), Sankhamul as a Research Intern. I am currently working on the research project “Molecular characterization of Biofilm generating population of non- multidrug resistant E. coli”. Microorganisms survive by attaching and growing on inanimate surfaces. Bioflms are extracellular polymers produced by these microorganisms that facilitate attachment and matrix formation. Research have shown a direct relation between biofilm- associated organisms and Multi- drug Antibiotic resistance. As Biofilms pose a great risk for public health, it has been studied extensively over past decades by researchers all over the world. Our research is primarily focused on finding a correlation between biofilm production and Multidrug resistance by taking Non-multidrug resistant organisms as control. I am working with non-MDR strains of E.coli obtained from swab cultures of patients from Medicare Hospital, Chabahil. Because of my interest in Cancer research, I am also assisting in the project “Analysis of EGFR mutations in non-small cell lung carcinoma patients visiting Nepal Cancer Hospital and Research Center.”.
At CHDS, I am learning about microscopic characterization techniques like gram staining, biochemical tests, Antibiotic Susceptibility test and molecular skills like DNA Isolation and PCR amplification. I have had the opportunity to get training on Transmission Assessment Survey (TAS) of Lymphatic Filariasis in Nepal. I was able to learn about public health and epidemiological research during the training and also developed my leadership skills through field works.
I am Pooja Shah, M.Sc. Microbiology Thesis Student from St. Xavier’s College, Maitighar, Kathmandu, Nepal. I have completed my under-graduation from Model Multiple College, Janakpur, Nepal.
Thesis title: Isolation and screening of bacteria with antibacterial activity isolated from soil sample of poultry farm. Started doing thesis work since Sep 2019.
Along with this, I have also been helping Prajita Thapa Magar (M.Sc. Microbiology Thesis Student) in her thesis work and other interns in Biofilm and EGFR projects on going at Centre for Health and Disease Studies, Nepal.
Rachel Aakriti Malla
Rachel Aakriti Malla is a high school junior at Broad Run High School in Ashburn, Virginia, United States of America. Rachel worked as a research intern during the summer of 2019 and her study entitled “Analysis of Oncogene, Anaplastic Lymphoma Kinase (ALK) in Human Cancers,” under the supervision of Dr. Roshan Lal Shrestha. This is a dry lab-based project in which Rachel used COSMIC, a cancer genomic bioinformatics tool.